BME 695N Lecture 16: Assessing drug efficacy at the single cell level - a podcast by James Leary

Outline:Introduction and overviewNanomedical treatment at the single cell level requires evaluation at the single cell levelFor evaluation purposes, does structure reveal function?The difficulty of anything but simple functional assaysThe need for assays which at least show correlation to functional activityQuantitative single cell measurements of one or more proteins per cell by flow and image/confocal cytometryCell surface measures of protein expression on live, single cellsHigh-throughput flow cytometric screening of bioactive compoundsChallenges of measuring protein expression inside fixed, single cellsWhen location is important 2D or 3D imaging is required to get spatial location of proteinsinside cellsQuantitative multiparameter phospho-specific flow cytometryAttempts to measure "functional proteins" by detecting phosphorylationExample of phospho-specific, multiparameter flow cytometryExample of measuring single cell gene silencing by phospho-specific flow cytometryQuantitative measures of gene expression – the promises and the realitiesIs gene expression at the single cell level really possible?Is it even useful to measure a single gene's changes?Gene arrays of purified cell subpopulationsRNA amplification techniques to attempt to perform single cell gene arrays

Further episodes of [Audio] BME 695N: Engineering Nanomedical Systems (Fall 2007)

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