Combining Light Sheet & Confocal Microscopy - a podcast by BiteSize Bio
from 2016-06-15T18:27:29
Living cells and organisms often suffer from the high light intensities that are used in conventional imaging. Light sheet microscopy reduces phototoxic effects and bleaching, by only illuminating a specimen in a single plane at a time whilst the signal is detected in a perpendicular direction. In combination with high-speed cameras for image acquisition, light sheet microscopy is a very gentle method to observe fast biological processes in sensitive organisms over an extended time period. By moving the sample along this plane, specimens are optically sectioned and imaged in 3D. These exciting possibilities led Nature Methods to cite light sheet imaging as their Method of the Year for 2014.
Learn more at http://bitesizebio.com/webinar/24339/the-best-of-both-worlds-combining-light-sheet-and-confocal-microscopy/
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